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VSP Team


VSP Team Members
Postdoctorates & Graduate Students
Undergraduate Interns

Dr. Lindsey du Toit

Lindsey du Toit grew up in the KwaZulu Natal province of South Africa, where she completed her undergraduate education at the University of Natal, Pietermaritzburg, in 1991 with a major in plant pathology. Lindsey went on to complete her MS (1995) and PhD (1998) degrees at the University of Illinois at Urbana-Champaign (UIUC), majoring in plant pathology. Her PhD dissertation was on field & epidemiological aspects of common smut of sweet corn caused by Ustilago maydis , under the direction of Dr. Jerald Pataky. During her stay in Illinois, Lindsey interned at the Plant Clinic of the UIUC for 5 growing seasons, supervised by Nancy Pataky. Lindsey's first position out of graduate school was the diagnostician for the Plant & Insect Diagnostic Lab at the Puyallup Research & Extension Center of Washington State University (WSU) from 1998 to 2000. Lindsey was then hired on as vegetable seed pathologist for WSU in August 2000, based at the Mount Vernon NWREC. The focus of the vegetable seed pathology program is development of a research and extension program on the biology and control of diseases of vegetable seed crops in Washington. In 2006, Lindsey was promoted to Associate Scientist & Extension Specialist E3.

Michael Derie
Agricultural Research Technologist III

Mike Derie, a native of Idaho, received his M.S. degree in plant science from the University of Idaho in 1989. He worked for 11 years at the WSU-Puyallup Research and Extension Center as a research technician in the vegetable seed pathology program under Dr. Gabrielson, where he focused on detection and control of black rot in crucifer seed. Mike joined the vegetable pathology program at Mount Vernon in 1997, with responsibility for carrying out laboratory-based research, making disease diagnoses, and helping to prepare manuscripts. In March 2001, Mike joined the vegetable seed pathology program at WSU-Mount Vernon NWREC. Mike is responsible for carrying out lab, field, and greenhouse trials on diseases of vegetable seed crops in Washington. Mike also isolates, identifies, and maintains many vegetable pathogens. In 2006 he celebrated his 20th year with WSU!
Louise Brissey
Agricultural Research Technologist II

Louise Brissey was born and raised in San Francisco, California. She graduated from California Polytechnic State University on the San Luis Obispo campus in 1972 with a B.S. degree in agriculture, majoring in ornamental horticulture. Louise has worked in an ornamental tissue culture lab in Skagit Co., and as assistant plant breeder for a flower seed company in CA. Louise worked in a time-slip position for the WSU Vegetable Seed Pathology (VSP) program for several years before she was hired on by the VSP program as an Ag. Research Tech. II in April 2005. Louise’s focus is on seed health assays and germination assays, but she assists with an array of lab, field, and greenhouse projects on diseases affecting vegetable seed crops. Louise is an avid gardener and is very involved in the Salal Chapter of the Washington Native Plant Society.

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Postdoctorates & Graduate Students

Leigh Ann Harrison
2006-2009 PhD dissertation: Management of Fusarium wilt in spinach seed production.

Leigh Ann was born in Odessa, TX, and lived in TX for 10 years, in France for 2 years, and in South Carolina for 12 years. In May 2004, Leigh Ann received a B.S. degree in Biological Sciences at Clemson University in Clemson, SC, where she earned four varsity letters as a member of the Lady Tiger soccer team. As an undergraduate in 2002, Leigh Ann was a member of the Cornell University Food Science Scholar Program under the supervision of Dr. Dennis Miller, in which she worked on a project assessing the bioavailability of iron powders intended for bread fortification. Leigh Ann’s undergraduate senior research project at Clemson, under Drs. Steve Jeffers and Melissa Riley, dealt with analyzing and developing fatty acid methyl ester profiles for Phytophthora cactorum. From July 2004 to October 2006, Leigh Ann did an MS degree in the WSU Department of Plant Pathology under the supervision of Dr. Tim Murray. Leigh Ann’s MS project was on the epidemiology of Wheat streak mosaic virus in perennial wheat, and screening for potential resistance to the virus in perennial wheat lines. Leigh Ann is continuing her education in the WSU Department of Plant Pathology as a PhD student under the direction of Dr. Lindsey du Toit. Leigh Ann’s PhD dissertation research is on management practices for Fusarium wilt in spinach seed production.

Jaime Cummings
2005 - 2007 MS thesis: Evaluation of organic seed treatments for control of seedling blight/damping-off pathogens of spinach.

Jaime was born in Binghamton, NY, and lived in NY for 20 years. Jaime attended Broome Community College to earn an A.S. degree in December 2002, with a focus on biology/chemistry. Jaime then attended the State University of West Georgia, where she completed courses towards her A.S., and focused on Spanish language studies. Jaime attended school in Cuernavaca, Morelos, Mexico for the summer of 2000-2001 - Jaime has completed 6 years of Spanish in high school, and 12 credits of Spanish at university level! She then earned a BS degree in wildlife biology/environmental forest biology in May 2004 from State University of New York College of Environmental Science and Forestry in Syracuse, NY. In 2004, Jaime ventured to the Amazon rainforest of Brasil to work on a tropical wildlife research project, followed by a position as research assistant on a small mammal research project at Virginia Tech, WV. Jaime worked as an outdoor environmental educator/naturalist, teaching biology and ecology courses to adults and children in 2004-2005. This was followed by a position in VA as a research assistant on a ground-nesting waterfowl research project for the College of William and Mary in 2005. Jaime started an MS degree in the WSU Department of Plant Pathology in July 2005, working in Lindsey du Toit’s vegetable seed pathology program on her MS thesis project, evaluating the efficacy of organic seed treatments for control of damping-off/seedling blight fungi on spinach.

Pablo Hernandez-Perez

2002 - 2005 MS thesis: Management of seedborne Stemphylium botryosum and Cladosporium variabile causing leaf spot of spinach seed crops in western Washington.

Pablo H. Palmández (previously Pablo Hernández Pérez) was born in Nayarit, México. In 1990, he graduated from the Universidad Autónoma Chapingo ( Mexico), where he studied the equivalent of a B.S. in agronomy with an emphasis in agricultural parasitology. His undergraduate thesis was titled: Exploración competitiva entre la maleza y el cultivo del rabanito (Raphanus sativus minor L.) en Chapingo, México (Study of competition between weeds and radishes (Raphanus sativus minor L.) in Chapingo, Mexico). Pablo studied English as a second language in Los Angeles, CA and in Yakima, Washington. In 1994, Pablo received a contract from the Plant Health Headquarters of the National Health, Food Safety and Quality Service of Mexico. He was sent to Washington State in 1995 by the Plant Health Headquarters to oversee adherence to the Work Plan for Apple Exportation from the United State to Mexico. In 1998, Pablo was contracted to work with the Washington State Horticultural Association as Scouting Coordinator for the Washington Pear IPM Project. Pablo received an M.S. degree in plant pathology from Washington State University in 2005 under the supervision of Dr. Lindsey du Toit. His M.S. thesis was entitled: Management of seedborne Stemphylium botryosum and Cladosporium variabile causing leaf spot of spinach seed crops in Western Washington. Pablo is a certified Spanish/English interpreter in Washington. He participated in the Spanish translation of the Orchard Monitoring Manual for Pests, Natural Enemies, and Diseases of Apple, Pear and Cherry, an illustrated guide for Washington State, compiled by Naná Simone. Pablo is currently working in insect genetics at the USDA - Yakima Agricultural Research Laboratory in Wapato, WA.

Publications from MS program:
Hernandez-Perez, P., and du Toit, L.J. 2006. Seedborne Cladosporium variabile and Stemphylium botryosum in spinach. Plant Disease 90:137-145.

du Toit, L.J., Derie, M.L., and Hernandez-Perez, P. 2005. Verticillium wilt in spinach seed production. Plant Disease 89:4-11.

du Toit, L.J., and Hernandez-Perez, P. 2005. Efficacy of hot water and chlorine for eradication of Cladosporium variabile, Stemphylium botryosum, and Verticilliumdahliae from spinach seed. Plant Disease 89:1305-1312. 

du Toit, L.J., Derie, M.L., and Hernandez-Perez, P. 2005. Evaluation of fungicides for control of leaf spot in spinach seed crops, 2004. Fungicide & Nematicide Tests60:V044.

du Toit, L.J., Derie, M.L., and Hernandez-Perez, P. 2005. Evaluation of yield loss caused by leaf spot fungi in spinach seed crops, 2004. Fungicide & Nematicide Tests 60:V047.

 du Toit, L.J., Derie, M.L., and Hernandez-Perez, P. 2004. Evaluation of fungicides for control of leaf spot in spinach seed crops, 2003. Fungicide & Nematicide Tests 59:V115.

Martin Chilvers
2002-2003 Postdoctorate project: Development of a real-time PCR seed assay for Botrytis spp. associated with neck rot of onion.

Martin Chilvers grew up in Tasmania, Australia, where he received a B.S. degree in agriculture, with honors in plant pathology (1998), from the University of Tasmania. In 2003, Martin received a Ph.D. degree in plant pathology from the University of Tasmania under Drs. Frank Hay and Calum Wilson. His PhD dissertation was entitled “Epidemiology of Botrytis spp. associated with neck rot of onion in northern Tasmania, Australia.” In December 2003, Martin took a research associate position at Washington State University co-supervised by Drs. Lindsey du Toit and Tobin Peever in Pullman, WA. The project was focused on development of a real-time quantitative PCR assay for detection of neck rot Botrytis spp. associated with onion seed. In early 2005, Martin began a position as research associate for Dr. Tobin Peever, working on various aspects of Ascochyta (Didymella) host specificity, epidemiology and genetics on chickpea.

Publications from postdoctorate:

Chilvers, M.I., du Toit L.J., Akamatsu, H., and Peever, T.L. 200X. A real-time, quantitative PCR seed assay for Botrytis spp. that cause neck rot of onion. Plant Disease: submitted August 2006.

Chilvers, M.I., and du Toit L.J. 2006. Detection and identification of Botrytis species associated with neck rot, scape blight, and umbel blight of onion. Plant Health Progress: in press.

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Undergraduate Interns

Cynthia Hansen, Western Washington University (summer 2007 intern)

Cynthia Hansen grew up in Bozeman, Montana and Fort Collins, Colorado. Having left the hot, dry summers of the Rocky Mountain west for the more temperate climate of Bellingham, Cynthia is entering her senior year at Western Washington University in fall 2007, working towards a BS in Cellular & Molecular Biology/Biochemistry. Cynthia is completing an internship with the WSU Vegetable Seed Pathology program for the summer of 2007, and is excited about the field of plant pathology. Cynthia is interested in continuing her studies with a graduate degree in plant sciences.

Alyse Douglas, Western Washington University (summer 2007 intern)

Alyse was born in 1986 and raised in North Bend, Washington. Alyse has also lived in California and Hawaii. Alyse attended Mount Si High School where she enrolled in Running Start through Bellevue Community College for her junior and senior years - she was in the top 10% of high school graduates in Washington State academically. Alyse will graduate at the end of fall 2007 at Western Washington University (WWU) where she is earning a B.S. in Cellular and Molecular Biology, with a minor in Chemistry. Alyse has completed a year as a Resident Advisor for Mathes Hall. Alyse spent two years doing organic chemistry research with Dr. Kriz at WWU, working on green laboratory procedures to be implemented in lab classes; and also completed a year as a Fellow Scientist for the GK-12 Catalysts for Reform project, where she helped create and implement an enquiry-based 6th grade science curriculum at Nooksack Valley Middle School. Alyse is completing an internship at WWU in conjunction with the Washington State University Mount Vernon NWREC, under the co-supervision of Dr. Marion Brodhagon (WWU molecular biologist), Dr. Lindsey du Toit (WSU vegetable seed pathologist), and Mike Derie (WSU agricultural research technologist). The internship project is on developing a quantitative molecular detection assay for Xanthomonas campestris pv. carotae, causal agent of bacterial blight of carrot, that differentiates DNA from viable (live) vs. nonviable (dead) cells of the pathogen. The internship is funded by the California Fresh Carrot Advisory Board. Alyse plans on continuing with a graduate education in infectious disease and eventually practicing medicine.

Jules Riske, Evergreen State College (spring 2007 intern)

Jules Riske grew up in rural Northern California, and now lives in Rockport, Washington in Eastern Skagit Co. At home with rough mountains and rivers canyons, she never thought about farming until she discovered the luscious, fertile Skagit River Valley. Having grown up in her father’s multi-acre garden and orchard, farming was as comfortable as an old pair of Carharts. Jules studied at the Evergreen State College, and will graduate with a degree in Ecological Agriculture in 2007. She interned and now works for Anne Schwartz, owner of Blue Heron Farm and Nursery in Rockport, WA. Jules serves on the board of the Tilth Producers of Washington, and when in the Skagit, she serves as secretary for the Sedro-Woolley Farmers Market board. Jules is involved in an 8-week internship in the Vegetable Seed Pathology program at the WSU Mount Vernon NWREC during April and May of 2007, assisting with field, greenhouse, and lab research on vegetable seed crop diseases.

Coen de Jong, Van Hall Institute in Leeuwaarden,
the Netherlands (fall 2006 intern)

Nicolaas “Coen” de Jong was born in Zuidbroek, the Netherlands, where his parents raise spinach stock seed in a greenhouse. Coen graduated from high school in 2005, and started college that year at the Van Hall Larenstein Institute, an agricultural college in the city of Leeuwaarden, the Netherlands. At the end of his first year of college, Coen completed a 10 week internship at Pop Vriend Seeds B.V. in Andijk, the Netherlands, where he worked primarily on parental lines in spinach seed production. Coen likes to work in his spare time, and has spent 5 years working part-time at Aardse Orchids B.V., a company that produces orchids in greenhouses. Coen completed a 9 week internship in the Vegetable Seed Pathology program at the WSU Mount Vernon NWREC in the fall of 2006, assisting with field, greenhouse, and lab research on vegetable seed crop diseases.

Blair Baber, Western Washington University (summer 2005 intern)

Blair Baber graduated from Western Washington University in December of 2006. At Western, he earned a B.S. degree in cellular biology with a minor in chemistry. As part of Blair’s degree, he completed an internship in the WSU vegetable seed pathology (VSP) program from June to November 2005. His main contribution in the VSP program was providing lab assistance for the onion Iris Yellow Spot Virus and carrot seed bacterial blight studies. Currently, Blair is working for US Biotek Laboratories in North Seattle as a lab technician. US BioTek uses ELISA (Enzyme-Linked-Immunosorbent-Assay) methods to identify and quantify IgG and IgE antibodies in human responses to foods, inhalants, herbs and spices.

Michael Picha, WSU (summer 2001 intern)

Michael Picha grew-up in Burlington, WA, and graduated from Washington State University in 2004 with a B.S. degree in Crop Science and a specialty in turf management. Michael completed an internship in the WSU vegetable seed pathology program in the summer of 2001, during which time he took responsibility for a fungicide trial for control of Stemphylium and C ladosporium leaf spots in spinach seed production. Michael is currently working as the Assistant Superintendent at the Glacier Club Golf Course in Durango, Colorado.

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